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Grand Challenges Canada

Bold Ideas with Big Impact®

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Development, Laboratory Testing and Field Validation of a Rapid Diagnostic Test for Ebola Virus Disease

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Start Date: 25/11/2015- End Date: 24/05/2015


Project Lead(s): Misaki Wayengera

Existing diagnostic technologies for detecting Ebola virus (EBOV) and Marburg virus (MARV) require laboratory facilities not present at the point of care (POC) in equatorial African villages, where these two Filoviruses cause rare but highly fatal viral hemorrhagic fevers (VHFs). 

As a first step towards the development of a rapid diagnostic test (RDT) based on the easy-to-use, cheap and yet reliable technology platform of lateral flow immunochromatography (aka paper strips or litmus test), three highly conserved epitopes in the filovirus surface or spike glycoprotein (Gp) were identified. 

These three epitopes along with their derivative antibodies (polyclonal-PAbs and or monoclonal-MAbs) were postulated to be potential biomarkers for the capture and detection of the two filovirus specific antibodies or antigens (Gp) in exposed and or infected humans. 

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    Program

    Transition to Scale - Scaling

    Institution

    Makerere University

    Institution Country

    Uganda

    Implementation Country

    Uganda

    Implementation Region

    Sub-Saharan Africa

    Priority

    Epidemics, Infectious Diseases, Traumatic Context

    Platform

    Diagnostics, Diagnostics / Diagnostics

Grand Challenges Canada’s programs are primarily undertaken with the financial support of the Government of Canada provided through Global Affairs Canada.

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