Detecting Multi-Drug Resistant TB with DNA Amplification by Destabilization: A New Simple Platform for Low-Income Countries

Project Lead(s): Julianne Gibbs-Davis

The accurate diagnosis of active TB, including drug-resistant forms, requires methods that can detect very small quantities of DNA from the TB bacteria in the infected person. Using destabilizing DNA that can be routinely made with common reagents, a small fragment of the TB DNA sequence can be amplified and detected without temperature cycling.